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Formation of eIF5A

The eukaryotic initiation factor 5A (eIF5A) is the only protein known to contain hypusine, an unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using  spermidine as the substrate.  Both eIF5A and deoxyhypusine synthase are essential genes.  Modulation of eIF5A has been linked to proliferation and cancer.  However, the function of eIF5A is unknown. We have first identified NAD+ as the cofactor for hypusine formation and isolated deoxyhypusine synthase, the key enzyme for hypusine formation.  We are interested in every aspects of eIF5A and hypusine formation in living world.

Features of eIF5A

Subcellular Localization of eIF5A

Subcellular localization of eIF5A by indirect immunofluorescence with two different fixation protocols.
 

Expression and localization of GFP-eIF5A in transiently transfected COS-7 cells.
 

Staining of eIF5A and calnexin or eIF5A and CRM1 by double immunofluorescence microscopy.
 

Expression and localization of GPK-eIF5A in transiently transfected COS-7 cells.
 

Expression and subcellular localization of GPK-tagged eIF-5A domain chimera proteins in living cells.
 

Heterokaryon analysis of the nuclear export of eIF5A
 

Effect of detergent extraction on the immunostaining patterns of eIF5A and GFP in the COS-7 cells.
 
eIF5A is an RNA Binding Protein
Interacting Partners of eIF5A

To View the Publications for the Hypusine Project, click here.